Fluorescence In Situ Hybridization (FISH)
Fluorescence in situ hybridization (FISH) can detect the presence, copy numbers and/or location of a DNA sequence within a set of metaphase chromosomes. FISH can detect monosomies, trisomies, and numerical sex chromosome abnormalities rapidly.
Red: Minor Satellite in Mouse ES cells
Green: Inserted Human gene
Multi-color Fluorescence In Situ Hybridization (m-FISH)
Multi-color Fluorescence in situ hybridization (m-FISH) can separate 24-color karyotyping that enable to find out the number and structural abnormalities of the entire chromosome in the cells. m-FISH can also identify the translocations and insersions in chromosome. The karyotyping is the process of pairing and ordering all the chromosomes of an organism in metaphase cells.
Karyotype of Human ES cell as visualized by Multi color fluorescence in situ hybridization (FISH)<46,XX>
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